00000ctm a22000003a 4500 UP-1685523046126152763 Buklod 20080924140230.0 aa r |||| u| ta 080924s xx d r |||| u| (iLib)UPMNL-00005473447 MED eng LG 995 1983 B3 Z34 Zafra, Racquel G. Studies on the effects of nutritional iron deficiency anemia on glycine matabolism on rats Zafra, Racquel. 87 leaves Theses--(MS in Biochemistry) University of the Philippines Manila Glycine metabolism was studied in iron-deficient anemic albino rats of the Sprague-Dawley strain. The rats showed increased serum levels of glycine amd serine. The 24-hour urinary excretion of glycine, but not the serine, was increased. Blood glucose levels increased one hour after intraperitoneal administration of glycine. A second hyperglycemic peak was observed between the third and fourth hour, a phenomenon that was not seen in the pair-fed controls. A hyperactive glucagon response might have caused this second peak and in turn would explain the decrease in liver glycogen levels observed five hours after glycine injection in the iron-deficient anemic group. Radioactive glycine labeled with C14 in the carboxylic carbon when injected intraperitpneally resulted in a higher rate of 14CO2 excretion during the 3-hour observation in the anemic iron-deficient rats. This suggests an active glycine cleavage system. The specific activity f glycogen three after glycine 1 14c injection was significantly lower in the anemic rats when compared to their controls. These findings indicate that the carboxyl group of glycine is poorly utilized for glycogen synthesis. In the liver, the activity of 3-phosphoglycerate dehydrogenase was increased and that of serine dehydratase was decreased. An increased activity of the dehydrogenase would result in an increased serine biosynthesis while a decrease in the activity of the dehydratase would result in a decreased catabolism of serine. The changes in the activities of these two enzymes concomitant with an active glycine-serine interconversion could account for the increased serine level in the serum and, at least in part, for the hyperglycinemia in anemic iron-deficient rats. D (-) lactate administration was followed consistently with the slowest clearance from the blood and the least hyperglycemic response, Glycogen deposition in the liver was very slight. Thus, conversion of glycine to D(-) lactate via the aminoacetone pathway is most probably not a major gluconeogenic pathway. Anemia, Iron-deficiency. Glycine. UPMNL MED LG 995 1983 B3 Z34 Thesis