00000nam a22000004i 4500 UP-1685523046126127196 Buklod 20230531143317.0 m o u cr ||||||||||| 230531s2016 ne u eng d 9780128092675 (OCoLC)952982268 UtOrBLW DSCI rda eng QD 96 F56 S56 2016eb Single-molecule enzymology fluorescence-based and high throughput methods edited by Maria Spies and Yann R. Chemla. Amsterdam, Netherlands Academic Press/Elsevier [2016] 1 online resource (xviii, 598 pages) illustrations (some color) text rdacontent computer rdamedia online resource rdacarrier Methods in enzymology volume 581 Includes bibliographical references and indexes. Chapter 1. Direct fluorescent imaging of translocation and unwinding by individual DNA helicases -- Chapter 2. Single-molecule imaging with one color fluorescence -- Chapter 3. Measuring membrane protein dimerization equilibrium in lipid bilayers by single-molecule fluorescence microsopy -- Chapter 4. Fluorescent labeling of proteins in whole cell extracts for single-molecule imaging -- Chapter 5. Quantifying the assembly of multicomponent molecular machines by single-molecule total internal reflection fluorescence microscopy -- Chapter 6. How to measure separations and angles between intramolecular fluorescent markers -- Chapter 7. Precisely and accurately inferring single-molecule rate constants -- Chapter 8. Quantification of functional dynamics of membrane proteins reconstituted in nanodiscs membranes by single turnover functional readout -- Chapter 9. Putting Humpty-Dumpty together: clustering the functional dynamics of single biomolecular machines such as the splicosome -- Chapter 10. Single-molecule FRET to measure conformational dynamics of DNA mismatch repair proteins -- Chapter 11. Single-molecule confocal FRET microscopy to dissect conformational changes in the catalytic cycle of DNA topoisomerases -- Chapter 12. Probing the conformational landscape of DNA polymerases using diffusion-based single-molecule FRET -- Chapter 13. Methods for investigating DNA accessibility with single nucleosomes -- Chapter 14. Single-molecule fluorescence studies of fast protein folding -- Chapter 15. Single-molecule multicolor FRET assay for studying structural dynamics of biomolecules -- Chapter 16. A multicolor single-molecule FRET approach to study protein dynamics and interactions simultaneously -- Chapter 17. Interferometric scattering microscopy for the study of molecular motors -- Chapter 18. Enzyme kinetics in femtoliter arrays -- Author index -- Subject index. IP-based subscription, on campus only. Access via Electronic Resources of the UPD University Library Website. Enzyme kinetics. Fluorescence microscopy. Fluorescence spectroscopy Congresses. Fluorescent probes Congresses. Luminescent probes Congresses. Electronic books. Spies, Maria editor. Chemla, Yann R. editor. Methods in enzymology volume 581. Available for University of the Philippines System via Science Direct. Also available remotely thru Open Athens: https://www.sciencedirect.com/bookseries/methods-in-enzymology/vol/581/suppl/C (viewed 25 May 2023) FO UPD DSCI Electronic Resource