Calorimetry - Feig, Andrew L. (Amsterdam, Netherlands Elsevier/ Academic Press, 2016)
Isothermal titration calorimetry measurements of metal ions binding to proteins -- Assess coupled protein folding and binding through temperature-dependent isothermal titration calorimetry -- Fragment-based screening for enzyme inhibitors using calorimetry -- Measuring multivalent binding interactions by isothermal titration calorimetry -- Calorimetric and spectroscopic analysis of the thermal stability of short duplex DNA-containing sugar and base-modified nucleotides -- Calorimetric quantification of cyclodextrin-mediated detergent extraction for membrane-protein reconstitution -- Extending ITC to kinetics with KinlTC -- Measuring the kinetics of molecular association by isothermal titration calorimetry -- Isothermal titration calorimetry to characterize enzymatic reactions -- Avoiding buffer interference in ITC experiments: a case study from the analysis of entropy-driven reactions of glucose-6-phosphate dehydrogenase -- ITC methods for assessing buffer/protein interactions from the perturbation of steady-state kinetics: a reactivity study of homoprotocatechuate 2,3-dioxygenase -- Modern analysis of protein folding by differential scanning calorimetry -- A guide to differential scanning calorimetry of membrane and soluble proteins in detergents -- Orthogonal methods for characterizing the unfolding of therapeutic monoclonal antibodies: differential scanning calorimetry, isothermal chemical denaturation, and intrinsic fluorescence with concomitant static light scattering -- IATC, DSC, and PPC analysis of reversible and multistate structural transition of cytochrome c -- The complementairy of the loop to the stem in DNA pseudoknots gives rise to local TAT base-triplets -- A high-throughput biological calorimetry core: steps to startup, run, and maintain a multiuser facility.
Includes bibliographical references and indexes.
Keywords: Calorimetry; Electronic books