00000ctm a22000003a 4500 UP-1685523046125456435 Buklod 20080527053857.0 a r |||| u| ta 080527s xx d r |||| u| (iLib)UPMNL-00003168914 UPMain eng LG 995 1977 P914 C38 Cauyan, Gil A. Survey for the presence of pathogenic fee-living amoeba in man Gil A. Cauyan. 44 leaves. Thesis (MS Public Health, Parasitoogy)--University of the Philippines, 1977 A survey was undertaken to determine whether free-living amoeba is present in man in the Philippines. A total of 2,101 subjects consisting of meningitic and meningoencephalitic patients, slum-dwellers, public elementary school children and persons living near a canal in Chicago St., Quezon City found positive for free-living amoebae pathogenicc to mice were imcluded in the survey. Nasal and throat swabs collected from the subjects were cultured in distilled water with Escherichia coli and examined after 7 days by direct microscopy for the presence of free-living amoeba. Not 1 out of 2,101 subjects was found positive for the free-living amoeba. With the last 50 samples, 25 each of nasal and throat swabs initially grown in distilled water with E. coli were pooled in 2 separate containers. After 7 days at room temperature these were centrifuged and the sediments were examined under direct microscopy for the presence of free-living amoeba. The remaining 25 each of nasal and throat swabs were also grown in distilled water with E. coli and after 7 days, these were centrifuged and the sediments were inoculated in agar seeded with E. coli. This is the 1st survey to be conducted in the Philippines to find out whether pathogenic free-living amoeba are present in the human population. Amoeba. Amoebida. UPMNL CPH LG 995 1977 P914 C38 Thesis