00000ctm a22000003a 4500 UP-1685523046125456024 Buklod 20080220132043.0 a r |||| u| ta 080220s2018 xx d r |||| u| (iLib)UPMNL-00001325302 UPDent rda eng LG 993.5 2018 D4 H44 Hemedes, Mary Rose A. author. An in vitro study on the antibacterial efficacy of methanolic extract of molave (Vitex parviflora A. juss) leaves against Streptococcus mutans Mary Rose A. Hemedes, Aerol Sedrick A. Mangalig and Rainier S. Rueda ; thesis adviser and co-auhtor, Jessica K. Rebueno Santos. Manila Department of Community Dentistry, University of the Philippines Manila c2018. 75 leaves color illustrations 28 cm atext rdacontent unmediated rdamedia volume rdacarrier INTRODUCTION: Studies have shown that molave (Vitex parviflora A. juss) has antibacterial properties against Staphylococcal microorganisms, but it has not been tested against oral pathogens such as Streptococcus mutans. The main objective of this study was to determine the antibacterial efficacy of molave leaves extract on the growth inhibition of Streptococcus mutans, in vitro. METHODS: An in vitro experimental design was used to determine the antibacterial efficacy of the plant extract through disk diffusion method. For cost-efficiency, the total sample size of 80 plates were reduced by placing the 5 test groups in one plate divided in 5 portions. The test was done in 18 replicates. 500 grams of fresh molave leaves from a single tree were collected and subjected to a methanolic extraction process. 50 mg/ml and 100 mg/ml molave extract, 0.12% chlorhexidine, distilled water, and 95% methanol were tested on 18 MHA plates inoculated with Streptococcus mutans. The plates were incubated for 48 hours in anaerobic conditions and the resulting zones of inhibition of each solution were measured with a millimeter ruler. The data were then analyzed through one-way ANOVA and Bonferroni test. RESULTS: The mean diameter of zones of inhibition produced by 100 mg/ml, 50 mg/ml concentration, and 0.12% chlorhexidine were 15.78, 11.63 mm, and 21.44mm respectively, while both the distilled water and 95% methanol did not inhibit bacterial growth. These results suggest that 100 mg/ml concentration has stronger antibacterial properties than 50 mg/ml concentration. Furthermore, both concentrations of the plant extract were relatively weaker compared to chlorhexidine. CONCLUSION: Molave leaves extract have the ability to inhibit the growth of Streptococcus mutans, under ideal circumstances, based on the diameter of zone of inhibition through disk diffusion method. Seminar Paper--Doctor of Dental Medicine--University of the Philippines Manila. Streptococcus mutans. Molave leaves extract. Mangaliag, Aerol Sedrick A. author. Ruedas, Rainier S. author. Rebueno Santos, Jessica K. thesis adviser and co-author. UP FI UPMNL DENT LG 993.5 2018 D4 H44 Thesis