Isotope labeling of biomolecules labeling methods - Kelman, Zvi. (Amsterdam, Netherlands Elsevier/Academic Press, 2015)
Robust high-yield methodologies for p2sH and p2sH/15N/13C labeling of proteins for structural investigations using neutron scattering and NMR -- Protein labeling in Escherichia coli with p2sH, 13C, and 15N -- Escherichia coli auxotroph host strains for amino acid-selective isotope labeling of recombinant proteins -- 19F-modified proteins and 19F-containing ligands as tools in solution NMR studies of protein interactions -- Biopolymer deuteration for neutron scattering and other isotope-sensitive techniques -- Production of bacterial cellulose with controlled deuterium-hydrogen substitution for neutron scattering studies -- Isotope labeling of proteins in Halobacterium salinarum -- Amino acid selective unlabeling in protein NMR spectroscopy -- Isotope labeling of eukaryotic membrane proteins in yeast for solid-state NMR -- Development of approaches for deuterium incorporation in plants -- Isotope labeling of proteins in insect cells -- Effective isotope labeling of proteins in a mammalian expression system -- Eschericia coli cell-free synthesis and isotope labeling of mammalian proteins -- Rapid biosynthesis of stable isotope-labeled peptides from a reconstituted in vitro translation system for targeted proteomics 347 -- Labeling of membrane proteins by cell-free expression -- Selective amino acid segmental labeling of multi-domain proteins -- Labeling monosaccharides with stable isotopes -- Stable isotope-labeled RNA phosphoramidites to facilitate dynamics by NMR -- In vivo, large-scale preparation of uniformly 15N- and site-specifically 13C-labeled homogrenous, recombinant RNA for NMR studies -- Cut and paste RNA for nuclear magentic resonance, paramagnetic resonance enhancement, and electron paramagnetic resonance structural studies.
Includes bibliographical references and indexes.
Keywords: Stable isotope tracers; Biomolecules -- Analysis; Electronic books