00000nmm a2200000 i 4500 UP-1685474870047060192 Buklod 20230510150152.0 m o u cr ||||||||||| 230404s2015 mau u eng d 9780128011232 (OCoLC)892454752 OCLCO DSCI rda eng QP 623 R53 2014eb Riboswitches as targets and tools edited by Donald H. Burke-Ageuero. Waltham, MA Elsevier/ Academic Press [2015] 1 online resource (xix, 423 pages, 24 unnumbered pages of plates) color illustrations text rdacontent computer rdamedia online resource rdacarrier Methods in enzymology volume 550 Includes bibliographical references and indexes. Design of transcription regulating riboswitches -- Ligand-dependent exponential amplification of self-replicating RNA enzymes -- Design of modular "plug-and-play" expression platforms derived from natural riboswitches for engineering novel genetically encodable RNA regulatory devices -- Integrating and amplifying signal from riboswitch biosensors -- Simple indentification of two causes of noise in an aptazyme system by monitoring cell-free transcription -- Engineering of ribosomal shunt-modulating eukaryotic ON riboswitches by using a cell-free translation system -- Live-cell imaging of mammalian RNAs with spinach2 -- In vitro analysis of riboswitch-spinach aptamer fusions as metabolite-sensing fluroescent biosensors -- Using spinach aptamer to correlate mRNA and protein levels in Eschrichia coli -- Monitoring mRNA and protein levels in bulk and in model vesicle-based artificial cells -- Design, synthesis and application of spinach molecular beacons triggered by strand displacement -- Using riboswitches to regulate gene expression and define gene function in mycobacteria -- Controlling expression of genes in the unicelleular alga Chlamydomonas reinhardtii with a vitamin-repressible riboswitch -- Conditional control of gene expression by synthetic riboswitches in Streptomyces coelicolor -- Engineering of ribozyme-based aminoglycoside switches of gene expression by In Vivo genetic selection in Saccharomyces cerevisiae -- Kinetic folding design of aptazyme-regulated expression devices as riboswitches for metabolic engineering -- Riboselector: riboswitch-based synthetic selection device to expedite evolution of metabolite-producing microorgansims -- Fluorescence assays for monitoring RNA-ligand interactions and riboswitch-targeted drug discovery screening -- Monitoring ribosomal frameshifting as a platform to screen anti-riboswitch drug candidates. IP-based subscription, on campus only. Access via Electronic Resources of the UPD University Library Website. Genetic regulation. Ligands (Biochemistry) Messenger RNA. RNA-protein interactions. Riboswitches. Molecular genetics Technique. Molecular genetics Methodology. Electronic books. Burke-Aguero, Donald H. editor. Methods in enzymology volume 550. Available for University of the Philippines System via Science Direct. Also available remotely for University of the Philippines System via Science Direct through. Click here to access thru Open Athens: https://www.sciencedirect.com/bookseries/methods-in-enzymology/vol/550/suppl/C (viewed 04 April 2023) FO UPD DSCI Electronic Resource