00000nmm a2200000 i 4500 UP-1685474870047060085 Buklod 20230510150748.0 m o u cr ||||||||||| 230404s2014 ne u eng d 9780128011850 (OCoLC)885225075 OCLCQ DSCI rda eng QH 442 U84 2014eb The use of CRISPR/Cas9, ZFNs, and TALENs in generating site-specific genome alterations edited by Jennifer A. Doudna, Erik J. Sontheimer. Amsterdam Elsevier/Academic Press [2014] 1 online resource (xx, 549 pages, 24 unnumbered pages of plates) illustrations (some color) text rdacontent computer rdamedia online resource rdacarrier Methods in enzymology volume 546 Includes bibliographical references and indexes. In vitro enzymology of Cas9 -- Targeted genome editing in human cells using CRISPR/Cas nucleases and truncated guide RNAs -- Determining the specificities of TALENs, Cas9, and other genome-editing enzymes -- Genome engineering with custom recombinases -- Genome engineering in human cells -- Genome editing in human stem cells -- Tagging endogenous loci for live-cell fluorescence imaging and molecule counting using ZFNs, TALENs, and Cas9 -- Genome editing using Cas9 nickases - Assaying break and nick-induced homologous recombination in mammalian cells using the DR-GFP reporter and Cas9 nucleases -- Adapting CRISPR/Cas9 for functional genomics screens -- The iCRISPR platform for rapid genome editing in human pluripotent stem cells -- Creating cancer translocations in human cells using Cas9 DSBs and nCas9 paired Nicks -- Genome editing for human gene therapy -- Generation of site-specific mutations in the rate genome via CRISPR/Cas9 -- CRISPR/Cas9-based genome editing in mice by single plasmid injection -- Imaging genomic elements in living cells using CRISPR/Cas9 -- Cas9-based genome editing in xenopus tropicalis -- Cas9-based genome editing in zebrafish -- Cas9-based genome editing in drosophila -- Transgene-free genome editing by germline injection of CRISPR/Cas RNA -- Cas9-based genome editing in arabidopsis and tobacco -- Mutliplex engineering of industrial yeast genomes using CRISPRm -- Protein engineering of Cas9 for enhanced function. IP-based subscription, on campus only. Access via Electronic Resources of the UPD University Library Website. Endonucleases Laboratory manuals. Genetic engineering Laboratory manuals. Genomics Laboratory manuals. Restriction enzymes, DNA Laboratory manuals. Electronic books. Doudna, Jennifer A. editor. Sontheimer, Erik J. editor. Methods in enzymology volume 546. Available for University of the Philippines System via Science Direct. Also available remotely for University of the Philippines System via Science Direct through. Click here to access thru Open Athens: https://www.sciencedirect.com/bookseries/methods-in-enzymology/vol/546/suppl/C (viewed 04 April 2023) FO UPD DSCI Electronic Resource