The use of CRISPR/Cas9, ZFNs, and TALENs in generating site-specific genome alterations - Doudna, Jennifer A.; Sontheimer, Erik J. (Amsterdam Elsevier/Academic Press, 2014)
In vitro enzymology of Cas9 -- Targeted genome editing in human cells using CRISPR/Cas nucleases and truncated guide RNAs -- Determining the specificities of TALENs, Cas9, and other genome-editing enzymes -- Genome engineering with custom recombinases -- Genome engineering in human cells -- Genome editing in human stem cells -- Tagging endogenous loci for live-cell fluorescence imaging and molecule counting using ZFNs, TALENs, and Cas9 -- Genome editing using Cas9 nickases - Assaying break and nick-induced homologous recombination in mammalian cells using the DR-GFP reporter and Cas9 nucleases -- Adapting CRISPR/Cas9 for functional genomics screens -- The iCRISPR platform for rapid genome editing in human pluripotent stem cells -- Creating cancer translocations in human cells using Cas9 DSBs and nCas9 paired Nicks -- Genome editing for human gene therapy -- Generation of site-specific mutations in the rate genome via CRISPR/Cas9 -- CRISPR/Cas9-based genome editing in mice by single plasmid injection -- Imaging genomic elements in living cells using CRISPR/Cas9 -- Cas9-based genome editing in xenopus tropicalis -- Cas9-based genome editing in zebrafish -- Cas9-based genome editing in drosophila -- Transgene-free genome editing by germline injection of CRISPR/Cas RNA -- Cas9-based genome editing in arabidopsis and tobacco -- Mutliplex engineering of industrial yeast genomes using CRISPRm -- Protein engineering of Cas9 for enhanced function.
Includes bibliographical references and indexes.
Keywords: Endonucleases -- Laboratory manuals; Genetic engineering -- Laboratory manuals; Genomics -- Laboratory manuals; Restriction enzymes, DNA -- Laboratory manuals; Electronic books