00000nmm a2200000 i 4500 UP-1685474870047059802 Buklod 20230510151403.0 m o u cr ||||||||||| 230324s2014 cau u eng d 9780124201194 (OCoLC)868642459 UKMGB DSCI rda eng QD 431.25 A53 L33 2014eb Laboratory methods in enzymology protein part C Eidted by Jon Lorsch. San Diego, California Elsevier/ Academic Press [2014] 1 online resource (xvii, 272 pages) illustrations text rdacontent computer rdamedia online resource rdacarrier Methods in enzymology volume 541 Includes bibliographical references and indexes. Section 1: Protein protocols/protein precipiation: TCA precipitation -- Section II: Protein protocols/protein pull-down methods: Coimmunoprecipitation of proteins from yeast -- Coupling antibody to cyanogen bromide-activated sepharose -- Analysis of protein-protein interactions by coimmunoprecipitation --Section III: Protein protocols/protein purification: Use and application of hydrophobic interaction chromatography for protein purification -- Hydroxyapatite chromatography: purification strategies for recombinant proteins -- Salting out of proteins using ammonium sulfate precipitation -- Using ion exchange chromatography to purify a recombinantly expressed protein -- Gel filtration chromatography (size exclusion chromatography) of proteins -- Section IV: Protein protocols/purification of membrane proteins: Expression and purification of membrane proteins -- Explantory chapter: choosing the right detergent -- Section V: Protein protocols/SDS PAGE: One-dimensional SDS-polyacrylamide gel electrophoresis (1D SDS-PAGE) -- Coomassie blue staining -- Silver staining of SDS-polyacrylamide gel -- Section VI: Protein protocols/Standard in vitro assays for protein-nucleic acid interactions: Standard in vitro assays for protein-nucleic acid interactions-gel shift assays for RNA and DNA binding -- Protein filter binding -- Section VII: Protein protocolstroubleshooting protein expression: Explanatory chapter: troubleshooting recombinant protein expression: general -- Explanatory chapter: troubleshooting protein expression: what to do when the protein is not soluble -- Section VIII: Protein protocols/Western blotting: Western blotting using chemiluminescent substrates. IP-based subscription, on campus only. Access via Electronic Resources of the UPD University Library Website. Proteins Analysis. Proteins Purification. Electronic books. Lorsch, Jon editor. Methods in enzymology volume 541. Also available remotely for University of the Philippines System via Science Direct through. Click here to access thru Open Athens: https://www.sciencedirect.com/bookseries/methods-in-enzymology/vol/541/suppl/C (viewed 24 March 2023) FO UPD DSCI Electronic Resource