Reconstituting the cytoskeleton - Vale, Ronald (Amsterdam, Netherlands Academic Press, 2014)
Actin filament dynamics using microfluidics -- Bacterial actin-like proteins: purification and characterization of self-assembly properties -- Quantitative analysis of microtubule self-assembly kinetics and tip structure -- Biochemical reconstitution of the WAVE regulatory complex -- Rotational movement of formins evaluated by using single-molecule fluorescence polarization -- Single-molecule studies of actin assembly and disassembly factors -- Assaying microtubule nucleation of the [gamma]-Tubulin ring complex -- Reconstituting dynamic microtubule polymerization regulation by TOG domain proteins -- Generation of differentially modified microtubules using In Vitro enzymatic approaches -- Engineering defined motor ensembles with DNA origami -- Construction and analyses of elastically coupled multiple-motor systems -- Reconstitution of cortical dynein function -- Reconstitution of microtubule-dependent organnelle transport -- Reconstituting the motility of isolated intracellular cargoes -- Reconstitution of contractile actomyosin arrays -- Directed actin assembly and motility -- In Vitro reconstitution of dynamic microtubules interacting with actin filament networks -- Measuring kinetochore-microtubule interaction In Vitro -- Micropattern-guided assembly of overlapping pairs of dynamic microtubules -- WAVE regulatory complex activation -- Dissecting principles governing actin assembly using yeast extracts -- Xenopus egg ctyoplasm with intact actin -- Glycogen-supplemented mitotic cytosol for analyzing xenopus egg microtubule organization -- Spindle assembly on immmobilized chromatin micropatterns.
Includes bibliographical references and indexes.
Keywords: Cytoskeleton; Microtubules; Muscle proteins; Electronic books