Laboratory methods in enzymology protein part A - Lorsch, Jon (San Diego, CA Elsevier/ Academic Press, 2014)
Practical steady-state enzyme kinetics -- Quantification of protein concentration using UV absorbance and Coomassie dyes -- Preparation of protein samples for mass spectrometry and N-terminal sequencing -- Lysis of mamalian and Sf9 cells -- In vivo [superscript 35 S]-methionine incorporation -- Pulse-chase analysis to measure protein degradation -- Labeling of a protein with fluorophores using maleimide derivitization -- Labeling a protein with fluorophores using NHS ester derivitization -- Protein derivitization-expressed protein ligation -- Protein biotinylation -- Small-scale expression of proteins in E. coli -- Protein expression-yeast - Recombinant protein expression in baculovirus-infected insect cells -- Single cell cloning of a stable mammalian cell line
Includes bibliographical references and indexes.
Keywords: Proteins -- Analysis; IP-based subscription, on campus only. -- Access via Electronic Resources of the UPD University Library Website