00000nmm a2200000 i 4500 UP-100084294495510740 Buklod 20230104071322.0 m o u cr ||||||||||| 221215t20132013ne u eng d 9780124186873 (OCoLC)858970409 OCLCO DSCI rda eng TP 248.24 L33 2013eb Laboratory methods in enzymology DNA edited by Jon Lorsch. Amsterdam, Netherlands Elsevier/ Academic Press [2013] 1 online resource (lvi, 348 pages) illustrations text rdacontent computer rdamedia online resource rdacarrier. Methods in enzymology volume 529 Includes bibliographical references and indexes. Explanatory chapter: PCR primer design -- Explanatory chapter: how plasmid preparation kits work -- Explanatory chapter: introducing exogenous DNA into cells -- Agarose gel electrophoresis -- Analysis of DNA by southern blotting -- Purification of DNA oligos by denaturing polyacrylamide gel electrophoresis (PAGE) -- Molecular cloning -- Rapid creation of stable mammalian cell lines for regulated expression of proteins using the Gatewayª recombination cloning technology and Flp-In-RExª lines -- Restrictionless cloning -- Isolation of plasmid DNA from bacteria -- Preparation of genomic DNA from bacteria -- Preparation of genomic DNA from saccharomyces cerevisiae -- Isolation of genomic DNA from mammalian cells -- Sanger dideoxy sequencing of DNA -- Preparation of fragment libraries for next-generation sequencing on the applied biosystems SOLiD platform -- Explanatory chapter: next generation sequencing -- Generating mammalian stable cell lines by electroporation --- Transient mammalian cell transfection with polyethylenimine (PEI) -- Site-directed mutagenesis -- PCR-based random mutagenesis -- Megaprimer method for mutagenesis of DNA -- Explanatory chapter: troubleshooting PCR -- Explanatory chapter: quantitative PCR -- General PCR -- Colony PCR -- Chemical transformation of yeast -- Transformation of E. coli via electroporation -- Transformation of chemically competent E. col IP-based subscription, on campus only. Access via Electronic Resources of the UPD University Library Website. Biotechnology Methodology. Molecular biology Methodology. Electronic books. Lorsch, Jon (ed.) editor. Methods in enzymology volume 529. Also available remotely for University of the Philippines System via Science Direct through. Click here to access thru Open Athens https://www.sciencedirect.com/bookseries/methods-in-enzymology/vol/529/suppl/C (viewed 15 December 2022) FO UPD DSCI Electronic Resource