Cloning and characterization of the gene encoding glyceraldehyde-3- phosphate dehydrogenase from coconut (Cocos nucifera L.)

Cloning and characterization of the gene encoding glyceraldehyde-3- phosphate dehydrogenase from coconut (Cocos nucifera L.)

Reverse Transcription-Polymerase Chain Reaction (RT-PCR) strategy was employed to clone the gene encoding glyceraldehyde-3-phosphate dehydrogenase (Gap) in coconut using a gene specific 20-mer primer pair. The sense primer had a 45.3 C melting temperature (Tm) and 50% Guanine-Cytosine (GC) content....

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Bibliographic Details
Published in:Philippine Agricultural Scientist, The (Formerly the Philippine Agriculturist) Vol. 91, no. 3 (Sep. 2008), 287-294
Main Author: Regalado, Ellen S.
Other Authors: Angeles, Jorge Gil C., Rodelas, Abigail Joy D., Garcia, Roberta N., Laurena, Antonio C., Tecson-Mendoza, Evelyn Mae
Format: Article
Language:English
Published: 2008
Subjects:
Coconut
Proteins
Cloning