Isolation of a CEL 1 Homolog in tomato (Solanum lycopersicum L.) fruit as a cost-effective endonuclease source for targeting induced local lesions in the genome (TILLING) analysis

Isolation of a CEL 1 Homolog in tomato (Solanum lycopersicum L.) fruit as a cost-effective endonuclease source for targeting induced local lesions in the genome (TILLING) analysis

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CEL 1, an endonuclease originally purified from celery, has been used in TILLING (Targeting Induced Local Lesions IN the Genome) analysis to cut the hairpin loop and single-strand DNA generated from heteroduplex of mutant and wild DNA molecules. However, one major limitation as with most mutation sc...

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Bibliographic Details
Published in:Philippine Journal of Science Vol. 148, no. 3 (Sep. 2019), 465-472
Main Author: Alcasid, Rochelle E.
Other Authors: Naredo, Maria Elizabeth B., Lantican, Darlon V., Galvez, Hayde F.
Format: Article
Language:English
Published: 2019
Subjects:
Tomato hybrids
Tomato products