Isozyme analysis of selected native and endemic Philippine freshwater fish species

In an attempt to establish the genetic relationship and to assess the genetic variation within and in between populations of selected endemic and native Philippine freshwater fishes, isozyme analysis was done using starch gel electrophoresis for four different enzyme systems namely; esterase, alkali...

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Bibliographic Details
Published in:Transactions of the National Academy of Science and Technology Vol. 33, no. 1 (Jul. 2011), 94
Main Author: Laude, Rita P.
Other Authors: Reyes, Roberto C., Rogelio, Diana Rose O., Ocampo, Pablo P.
Resource Type: Article
Language:English
Published: 2011
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Summary:In an attempt to establish the genetic relationship and to assess the genetic variation within and in between populations of selected endemic and native Philippine freshwater fishes, isozyme analysis was done using starch gel electrophoresis for four different enzyme systems namely; esterase, alkaline phosphatase, malic enzyme and á-glycerophosphate dehydrogenase. A total of 285 fish individuals were used that include Leiopotherapon plumbeus ("Ayungin"), Glossogobius celebius ("Biyang Bato"), Glossogobius giuris ("Biyang Bato") and Ophieleotris aporos ("Bangayngay"). The crude protein extracts were loaded onto the starch gel and the electrophoretic run followed. After the run was finished, the gel was sliced into four and each slice was soaked in a specific enzyme staining solution. The resolved bands were then scored and the relative mobility (Rf) values were computed for each band. Preliminary results include the banding patterns observed for each population and the temporarily assigned presumptive loci and alleles based on the Rf values. The loci and alleles assigned are still temporary until all the bands are confirmed for their presence and position which is now underway. These loci and alleles will be the basis for the statistical analysis using POPGENE32 software. However, to have a more effective analysis, four more enzyme systems namely: acid phospatase, lactate dehydrogenase, phosphogluconic acid, and isositric dehydrogenase will be added.