00000cab a22000004cb4500 IPP-00000277201 IPP 20190208161805.0 190208s2011 xx d | ||r |||||eng|| eng Yadao-Dela Cruz, Reggie Selection of a suitable internal control gene for relative RT PCR-based expression analysis in normal and mutant ‘Makapuno’ endosperms of coconut (Cocos nucifer L.) 2011 Makapuno is an abnormal coconut with over-proliferating endosperm that is softer and fluffy due to galactomannan accumulation. To perform relative RT-PCR for expression analyses of important genes possibly involved in the makapuno phenomenon, an internal control of genes was initially determined for normalization purposes. Three genes were studied, namely, glyceraldehydes-3-phosphate dehydrogenase (GAPDH), enolase and actin. GAPDH and enolase were found to have considerably varied expression level in all the samples and are not recommended to be used as internal control genes. The actin gene has a uniform detectable expression in all the samples studied and is recommended for use as reference gene for normal coconut and mutant makapuno solid endosperm samples at stages 5-6, 6-7 and 7-8 months. Macapuno (Coconut) Laude, Rita P. Diaz, Ma. Genaleen Q. Laurena, Antonio C. Mendioro, Merlyn S. Mendoza, Evelyn Mae T. Transactions of the National Academy of Science and Technology Vol. 33, no. 1 (Jul. 2011), 86 UPD DMLP Article FI